Wound Healing Research
Meta-Analyses/Reviews
Woodruff, L., Bounkeo, J., Brannon, W., Dawes, K. et al (2004). The efficacy of laser therapy in wound repair: a meta-analysis of the literature. Photomedicine and Laser Surgery 22(3):2417.
Schindl, A., Schindl, M., Pernerstorfer-Schoen, H., Schindl, L. (2001). Low intensity laser therapy in wound healing - A review with special respect to diabetic angiopathies. Acta Chirurgica Austriaca. 33(3):132-137.
Nicolette Houreld, Heidi Abrahamse (2005). Low-level laser therapy for diabetic foot wound healing The Diabetic Foot, Winter 2005.
Bryant, Rebecca Low-level laser outshines other modalities for wound treatment: correct dosimetry is critical, researcher says. (Lasers and beyond) Dermatology Times, 6/15/2004.
Clinical Studies
Minatel, D.G., Frade, M.A.C, Franca S.C. and Enwemeka, C. S. (2009). Phototherapy Promotes Healing of Chronic Diabetic Leg Ulcers that Failed to Respond to Other Therapies. Lasers in Surgery and Medicine 41:433-441.
Herascu N; Velciu B; Calin M; Savastru D; Talianu C (2005). Low-level laser therapy (LLLT) efficacy in post-operative wounds. Photomedicine and Laser Surgery 23 (1):70-3. Abstract: Objective: The aim of this paper was to investigate the efficacy of low-level laser radiation (LLLR) with wavelength of 904 nm on the stimulation of the healing process of postoperative aseptic wounds (early scar). Background Data: Low-level laser therapy (LLLT) has been increasingly used to treat many disorders, including wounds. However, despite such increased clinical usage, there is still controversy regarding the efficacy of this wound treatment in curent clinical practice. Methods: LLLT has been used to treat cutting plague in the right instep and on the left foot. Both resulted from sutured wounds. The clinical evaluation by semiquantitative methods is presented. Results: Clinical evaluation showed that the healing process of these postoperatively treated wounds has occurred and that the functional recovery of the patients (i.e., return to their ordinary life) was faster than without treatment. Conclusion: LLLR with wavelength of 904 nm to stimulate postoperative aseptic wounds (early scar) is efficient in both cases of cutting plague.
Al-Watban, F. & Zhang, X. (2004). The comparison of effects between pulsed and CW lasers on wound-healing. J Clin Laser Med Surg 22(1):15-8.
Z; Ivankovich AD; Depolo A., Simunovic (2004). Wound healing of animal and human body sport and traffic accident injuries using low-level laser therapy treatment: a randomized clinical study of seventy-four patients with control group. Journal of Clinical Laser Medicine & Surgery: 18 (2):67-73.
Maiya GA; Kumar P; Rao L ( ). Effect of low intensity helium-neon (He-Ne) laser irradiation on diabetic wound healing dynamics. Photomedicine and Laser Surgery 23 (2):187-90
Lagan-K-M, Mc-Donough-S-M, Clements-B-A, Baxter-G-D (2000). A case report of low intensity laser therapy (LILT) in the management of venous ulceration: potential effects of wound debridement upon efficacy. Journal of Clinical Laser Medicine & Surgery 18 (1): 15-22.
Hopkins, J.T., McLoda, T.A., Seegmiller, J.G. and Baxter, G.D. (2004). Low-Level Laser Therapy Facilitates Superficial Wound Healing in Humans: A Triple-Blind, Sham-Controlled Study. Journal of Athletic Training 2004:39(3):223-229.
Hirschl, M., Katzenschlager, R., Ammer, K., Melnizky, P. et al (2002). Double blind, randomised, placebo controlled low level laser therapy study in patients with primary Raynaud’s phenomenon. VASA 31(2):91-4.
Al Awami M, Schillinger M, Gschwandtner M E et al (2001). Low level laser treatment of primary and secondary Raynaud's phenomenon. VASA 30 (4): 281-284.
Hirschl, M., Katzenschlager, R., Francesconi, C., Kundi, M. (2004). Low level laser therapy in primary Raynaud’s phenomenon. Journal of Rheumatology 31(12):2408-12.
Fugimaki, Y., Shimoyama, T., Liu, Q. et al (2003). Low level laser irradiation attenuates production of reactive oxygen species by human neutrophils. J Clin Laser Med Surg 21 (3): 165-70.
Verdote-Robertson-R, Munchua-M-M, Reddon-J-R (2000). The use of low intensity laser therapy (LILT) for the treatment of open wounds in psychogeriatric patients: A pilot study. Physical and Occupational Therapy in Geriatrics 18(2):1-19.
V. M. Lisienko, O. J. Menjajlenko ( ). Therapeutic laser in treatment of trophic ulcers (TU) of venous aetiology. The Ural State Medical Academy.
Waynant, R., (1998). US Food & Drug Administration: results from a preliminary wound healing trial. Notes from a presentation at The 2nd Congress of The world Assoc. for Laser Therapy, Kansas, MO, USA, Sept. 2.5 1998.
Hawkins, D., Houreld, N., & Abrahamse, H. ( ). Low Level Laser Therapy (LLLT) as an Effective Therapeutic Modality for Delayed Wound Healing. Faculty of Health, University of Johannesburg, Johannesburg, 2028, South Africa
Djavid, G. E. (2006 ). Evaluation of low-level-laser irradiation in the healing of diabetic foot wounds: a randomized controlled trial, Iranian Ctr. for Medical Laser (Iran) and Tehran Univ. of Medical Sciences (Iran) [6140-21]
Mirko Hirschl, Reinhold Katzenschlager, Claudia Francesconi, And Michael Kundi (2004). Low Level Laser Therapy in Primary Raynaud's Phenomenon — Results of a Placebo Controlled, Double Blind Intervention Study From the Department of Angiology, Hanusch Hospital, Vienna; and the Institute of Environmental Health, Medical University of Vienna, Vienna, Austria.
Minar E. Low level laser therapy for treatment of primary and secondary Raynaud's phenomenon. Department of Medical Angiology, University of Vienna, Austria.
mahdi.al-awami@akh-wien.ac.at
Experimental Studies
Pessoa, E., Melhado, R., Theodoro, L., Garcia, V. (2004). A histologic assessment of the influence of low intensity laser therapy on wound healing in steroid treated animals. Photomedicine & Laser Surgery 22(3):199-203.
Z; Ivankovich AD; Depolo A., Simunovic (2004). Wound healing of animal and human body sport and traffic accident injuries using low-level laser therapy treatment: a randomized clinical study of seventy-four patients with control group. Journal of Clinical Laser Medicine & Surgery: 18 (2):67-73.
Kawalec, J., Hetherington, J., Pfennigwerth, C. et al (2004). Effect of a diode laser on wound healing by using diabetic and nondiabetic mice. Journal of Foot and Ankle Surgery 43.
Lanzafame RJ; Stadler I; Coleman J; Haerum B; Oskoui P; Whittaker M; Zhang RY (2004) Temperature-controlled 830-nm low-level laser therapy of experimental pressure ulcers. Photomedicine and Laser Surgery 22 (6): 483-8.
Mendez TM; Pinheiro AL; Pacheco MT; Nascimento PM; Ramalho LM (2004) Dose and wavelength of laser light have influence on the repair of cutaneous wounds. Journal of Clinical Laser Medicine & Surgery 22 (1): 19-25.
Silva, J., Lacava, Z., Kuckelhaus, S., Silva, L. et al (2004). Evaluation of the use of low level laser and photosensitizer drugs in healing. Lasers in Surgery & Medicine 34(5): 451-7.
Mirsky, N., Krispel, Y., Shoshany, Y., Maltz, L., Oron, U. (2002). Promotion of angiogenesis by low energy laser irradiation. Antioxid Redox Signal 4(5):785-790.
Barbosa A M, Villaverde A B, Guimarães-Sousa L I, Soares A M, Zamuner S F, Cogo J C, Zamuner S R (2010). Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom. Journal of Venomous Animals and Toxins including Tropical Diseases 16 (3). Abstract: The prominent myotoxic effects induced by Bothrops jararacussu crude venom are due, in part, to its polycationic myotoxins, BthTX-I and BthTX-II. Both myotoxins have a phospholipase A2 structure: BthTX-II is an active enzyme Asp-49 PLA2, while BthTX-I is a Lys-49 PLA2 devoid of enzymatic activity. In this study, the effect of low-level laser therapy (LLLT), 685 nm laser at a dose of 4.2 J/cm2 on edema formation, leukocyte influx and myonecrosis caused by BthTX-I and BthTX-II, isolated from Bothrops jararacussu snake venom, was analyzed. BthTX-I and BthTX-II caused a significant edema formation, a prominent leukocyte infiltrate composed predominantly by neutrophils and myonecrosis in envenomed gastrocnemius muscle. LLLT significantly reduced the edema formation, neutrophil accumulation and myonecrosis induced by both myotoxins 24 hours after the injection. LLLT reduced the myonecrosis caused by BthTX-I and BthTX-II, respectively, by 60 and 43%; the edema formation, by 41 and 60.7%; and the leukocyte influx, by 57.5 and 51.6%. In conclusion, LLLT significantly reduced the effect of these snake toxins on the inflammatory response and myonecrosis. These results suggest that LLLT should be considered a potential therapeutic approach for treatment of local effects of Bothrops species venom.
In Vitro Studies
Webb, C. & Dyson, M. (2003). The effect of 880nm low level laser energy on human fibroblast cell numbers: a possible role in hypertrophic wound healing. Journal of Photochemistry & Photobiology B(?)Biol (or Vol?) 70: 39-44.
Nussbaum, E., Lilge, L. & Mazzulli, T. (2003). Effects of low level laser therapy of 810nm upon in vitro growth of bacteria. J Clin Laser Med Surg 21(5): 283-90.
Medrado, A., Pugliese, L., Reis, S., Andrade, Z. (2003). Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Lasers in Surgery & Medicine 32(3): 239-44.
Stadler I, Lanzafame R J, Evans R, et al (2001). 830-nm irradiation increases the wound tensile strength in a diabetic murine model. Lasers in Surgery and Medicine. 28 (3): 220-226.
Webb C, Dyson M, Lewis WH. (1998). Stimulatory effect of 660 nm low level laser energy on hypertrophic scar-derived fibroblasts: possible mechanisms for increase in cell counts. Lasers Surg Med. 22(5):294-301.
Ph.van der Veen, Y de Rop, P. Lievens ( ). The influence of IR-Laser on the proliferation of fibroblasts: An in-vitro study.
Lievens P, van der Veen P ( ). Wound Healing Process: Influence Of LLLT On The Proliferation Of Fibroblasts And On The Lymphatic Regeneration. Department of Rehabilitation Research Vrije Universiteit Brussel, Brussels, Belgium